HaeIII from New England Biolabs

Product Specs
ItemHaeIII
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberR0108L
Quantity15000 units
Concentration10-50 u/µL
ApplicationsDNA Manipulation
Brand/TrademarkNew England Biolabs Inc (NEB)
TypeRestriction Enzyme
Temperature-20 C

Sequence

GG/CC

Add to Compare List

New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Nuclear growth and import can be uncoupledbioRxivApril 25, 2023Pan Chen, Sampada Mishra, Daniel L. Levy(NEB, M0247S), 0.2 U/μL EcoRI (NEB, R0101S), 0.2 U/μL XhoI (NEB, R0101S), or 0.5 U/μL HaeIII (NEB, R0108S) was added to X. laevis egg extract after nuclear assembly. WGA (Sigma, L9640) and 150-kDa FITC-dextran

(2) The conserved histone chaperone Spt6 is strongly required for DNA replication and genome stabilityCell ReportsMarch 28, 2023Catherine L.W. Miller, Fred Winston(IAA),Sigma,12886 RnaseH,NEB,M0297L AccI,NEB,R0161S EcoRV,NEB,R3195S NcoI,NEB,R3193S HaeIII,NEB,R0108S BsrG1,NEB,R3575S SigmaFAST protease inhibitor cocktail tablets,Sigma,S8820-20TAB RNaseA/T1 Mix,Thermo,EN0551 Benzonase,Sigma,E1014-5KU Dynabeads

(3) Characterization of a Novel Thermostable DNA Lyase Used To Prepare DNA for Next-Generation SequencingChemical research in toxicologyFebruary 20, 2023Baljinnyam T, Conrad JW, Sowers ML, Chang-Gu B, Herring JL, Hackfeld LC, Zhang K, Sowers LC.Mismatch-specific endonuclease I (MSE, #M0678S) and restriction enzymes HpaII (#R0171S) and HaeIII (#R0108S) were also purchased from NEB. Human 8-oxoguanine DNA glycosylase (hOGG1, #NBP1-45318-0.1mg) and DNA

(4) Genome concentration, characterization, and integrity analysis of recombinant adeno-associated viral vectors using droplet digital PCRPLOS ONEJanuary 25, 2023Andrew Prantner, Dianna MaarU of restriction enzyme. The female DNA and synthetic gBlock DNA used HaeIII (New England Biolabs, R0108L) and the plasmid DNA used either HindIII (New England Biolabs, R0104L) or MspI (New England Biolabs,

(5) STRAIGHT-IN enables high-throughput targeting of large DNA payloads in human pluripotent stem cellsCell Rep MethodsOctober 24, 2022Blanch-Asensio A, Grandela C, Brandão KO, de Korte T, Mei H, Ariyurek Y, Yiangou L, Mol MPH, van Meer BJ, Kloet SL, et al.,New England Biolabs,Cat#R0150S NsiI,New England Biolabs,Cat#R0127S Esp3I,New England Biolabs,Cat#R0734S HaeIII,New England Biolabs,Cat#R0108S MseI,New England Biolabs,Cat#R0525S BpiI,ThermoFisher,Cat#ER1011 FastDigest Eco31I,ThermoFisher,Cat#FD0293 T4 DNA Ligase

(6) The Rise and Fall of Billionaire siRNAs during Reproductive Development in RicePlants (Basel)July 28, 2022Lili Wang, Dachao Xu, Longjun Zeng, Dong-Lei Yangare listed in Table S3. 4.7. Chop-PCR A 1 μg quantity of genomic DNA was digested by HaeIII (NEB, R0108S) in a 30 μL reaction mixture at 37 °C for 16 h. A 2 μL volume of the digested DNA was used as a template

(7) MyoD is a 3D genome structure organizer for muscle cell identityNature CommunicationsJanuary 11, 2022Ruiting Wang, Fengling Chen, Qian Chen, Xin Wan, Minglei Shi, Antony K. Chen, Zhao Ma, Guohong Li, Min Wang, Yachen Ying, et al.X-100, and the genome was digested into blunt-end fragments with 5 µl HaeIII (10 U/µl, NEB, Cat.N: R0108L) in 81.5 µl H2O, 10 µl 10 × NEB buffer 2, 2.5 µl 20% Triton X-100, and 1 µl BSA at 37 °C for at least

(8) Repeated strand invasion and extensive branch migration are hallmarks of meiotic recombinationMolecular CellOctober 1, 2021Jasvinder S. Ahuja, Catherine S. Harvey, David L. Wheeler, Michael Lichten,New England Biolabs,R0194L SpeI-HF,New England Biolabs,R3133L XcmI,New England Biolabs,R0533L HaeIII,New England Biolabs,R0108L NdeI,New England Biolabs,R0111S DdeI,New England Biolabs,R0175S PstI,New England Biolabs,R0140S PvuII,New England Biolabs

(9) Smart-RRBS for single-cell methylome and transcriptome analysisNature ProtocolsAugust 1, 2021Hongcang Gu et al.cat. no. P7204S) MspI (New England Biolabs, cat. no. R0106L) HaeIII (New England Biolabs, cat. no. R0108S) Klenow fragment (3′→5′ exo-; New England Biolabs, cat. no. M0212L) Deoxynucleotide solution set,

(10) Emergent canine visceral leishmaniasis in Argentina: Comparative diagnostics and relevance to proliferation of human diseasePLOS Neglected Tropical DiseasesJuly 19, 2021Kyoko Fujisawa et al.1 cycle of 72°C for 10 mins. Following the ITS1 PCR, half of the reaction was digested with HaeIII (R0108S, New England Biolabs) in supplied 1x buffer, and then electrophoresed on 3% or 3.5% agarose gel. Additionally,

(11) PIE-1 SUMOylation promotes germline fates and piRNA-dependent silencing in C. eleganseLifeMay 18, 2021Heesun Kim, Yue-He Ding, Shan Lu, Mei-Qing Zuo, Wendy Tan, Darryl Conte, Meng-Qiu Dong, Craig C Mello,New England Biolabs,Cat# R3131S, "Peptide, recombinant protein",HaeIII (screen for G56R),New England Biolabs,Cat# R0108S, "Peptide, recombinant protein",Alt-R S.p. Cas9 Nuclease V3,Integrated DNA Technologies (IDT),Cat#

(12) Semisynthesis of site-specifically succinylated histone reveals that succinylation regulates nucleosome unwrapping rate and DNA accessibilityNucleic Acids ResearchSeptember 25, 2020Yihang Jing, Dongbo Ding, Gaofei Tian, Ka Chun Jonathan Kwan, Zheng Liu, Toyotaka Ishibashi, Xiang David Liwas loaded by stepwise dialysis. The HaeIII enzyme digestion (final concentration at 1.5 U/μL, NEB R0108S) was carried out in digestion buffer (10 mM Tris, pH 7.9, 10 mM MgCl2, 150 mM NaCl, 20 μg/mL BSA) with

(13) Quantitative Assessment of Parental Somatic Mosaicism for Copy‐Number Variant (CNV) DeletionsCurrent Protocols in Human GeneticsJune 1, 2020Qian Liu, Christopher M. Grochowski, Weimin Bi, James R. Lupski, Paweł Stankiewiczfor the fragmentation of the template >66 ng per reaction: HaeIII (New England Biolabs, NEB, cat. no. R0108S), MseI (NEB, cat. no. R0525S), AluI (NEB, cat. no. R0137S), HindIII (NEB, cat. no. R3104S), and CviQI

(14) Efficient and Reproducible Multigene Expression after Single-Step Transfection Using Improved BAC Transgenesis and Engineering ToolkitACS Synthetic BiologyMay 15, 2020Binhui Zhao, Pankaj Chaturvedi, David L. Zimmerman, Andrew S. Belmontfollowing reagents: AluI, DpnI, HaeIII, MseI, MspI, RsaI (New England Biolabs, Cat. # R0137S, R0176S, R0108S, R0525S, R0106S, R0167S, respectively) and CutSmart Buffer (New England Biolabs); Terminal Deoxynucleotidyl

(15) Sci-Hi-C: A single-cell Hi-C method for mapping 3D genome organization in large number of single cellsMethodsJanuary 1, 2020Vijay Ramani, Xinxian Deng, Ruolan Qiu, Choli Lee, Christine M. Disteche, William S. Noble, Jay Shendure, Zhijun Duanwell Water,4.3 μl 10% Triton X-100,0.1 μl "AluI (10 unit/ μl, NEB R0137L)",0.3 μl "HaeIII (10 unit/ μl, NEB R0108L)",0.3 μl

(16) Activation of Oncogenic Super-Enhancers Is Coupled with DNA Repair by RAD51Cell ReportsOctober 15, 2019Idit Hazan, Jonathan Monin, Britta A.M. Bouwman, Nicola Crosetto, Rami I. AqeilanProteins" ,, TOP1 Ab,Abcam,Cat# ab3825 TOP1 Ab,Abcam,Cat# ab85038 HaeIII,New England Biolabs,Cat# R0108S T4 DNA Ligase,New England Biolabs,Cat# M0202M Truncated RNA Ligase,New England Biolabs,Cat# M0242S T4 Polynucleotide Kinase

(17) Lead, Cadmium and Zinc Phytotoxicity Alter DNA Methylation Levels to Confer Heavy Metal Tolerance in WheatInternational Journal of Molecular SciencesSeptember 20, 2019Sarfraz Shafiq, Qudsia Zeb, Asim Ali, Yasar Sajjad, Rashid Nazir, Emilie Widemann, Liangyu LiuUSA) for CG DNA methylation, and with AluI (New England Biolabs, Ipswich, MA, USA USA) and haeIII (R0108S New England Biolabs, Ipswich, MA, USA) for CHH/CHG methylation. Equal amount of digested and undigested

(18) Commensal Neisseria kill Neisseria gonorrhoeae through a DNA-dependent mechanism in miceCell Host & MicrobeAugust 1, 2019Won Jong Kim, Dustin Higashi, Maira Goytia, Maria A. Rendón, Michelle Pilligua-Lucas, Matthew Bronnimann, Jeanine A. McLean, Joseph Duncan, David Trees, Ann E. Jerse, et al.RESOURCE,SOURCE,IDENTIFIER Enzymes,Enzymes,Enzymes HaeIII,"New England Biolabs (NEB) (Ipswich, MA, USA)",Cat# R0108S NgoIV,NEB,Cat# R0564S NlaIV,NEB,Cat# R0126S AsiSI,NEB,Cat# R0630S BamHI,NEB,Cat# R0136S NotI,NEB,Cat#

(19) Pervasive Chromatin-RNA Binding Protein Interactions Enable RNA-Based Regulation of TranscriptionCellJune 27, 2019Xiao R, Chen JY, Liang Z, Luo D, Chen G, Lu ZJ, Chen Y, Zhou B, Li H, Du X, et al.ligase,Epicenter,CL9021K APE 1,NEB,M0282S SYBR Gold Nucleic Acid Gel Stain,Thermo,S-11494 HaeIII,NEB,R0108L Dynabeads M-280 Streptavidin,Thermo,11205D I-Block Protein-Based Blocking Reagent,Thermo,T2015 Salmom

(20) mTOR inhibitors may benefit kidney transplant recipients with mitochondrial diseasesKidney InternationalFebruary 1, 2019Simon C. Johnson et al.and analyzed by restriction digestion using HaeIII according to the manufacturer’s specifications (R0108S; NEB, Ipswich, MA). Cleaved and uncleaved products were run on a 4% to 20% tris/borate/EDTA polyacrylamide

Be the First to Write a Review!

HaeIII

HaeIII from New England Biolabs

Sequence

New England Biolabs

Citations (25)

Reviews

HaeIII from New England Biolabs

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map